This study included 110 fish, and the proportion of isolated bacteria was determined to be 85% in the intestines, 25% in the skin, and 55% in the liver, with Aeromonas spp. making up the remaining 25%. Oxytetracycline (OX30), with a concentration of 4.5 ± 0.10mm, proved to be the most effective antibiotic in suppressing Aeromonas according to antibiotic sensitivity testing. In contrast, tetracycline (TI30) yielded unimpressive results. Antibiotic efficacy was enhanced by adding cell-free supernatants (CFS) derived from lactic acid bacteria. The data indicated that the optimal concentration of CFS was 50mg/ ml, suggesting that lactic acid bacteria could be a valuable tool in combating antibiotic resistance and improving fish health. In conclusion, this study suggests that lactic acid bacteria may help inhibit the growth of harmful Aeromonas strains, highlighting the need to reconsider current approaches to managing drug-resistant bacteria.