409052

Estimation of the sensitivity of a novel technique for the detection of E. coli O157 in non-heat-treated milk

Article

Last updated: 09 Mar 2025

Subjects

-

Tags

Animal Health

Abstract

Escherichia coli O157: H7 is an important pathogen that causes fatal infections. It has been the cause of several outbreaks in different areas world-wide and is associated with high mortality and morbidity. Multiple procedures were adopted to detect this serotype, but conventional culture techniques represent the gold standard. In this study we estimated peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) technique for the rapid detection of E. coli O157:H7. PNA-FISH technique was performed for 50 strains; 20 were E. coli O157 H7, 20 were E. coli O157 non-H7 strains, and 10 strains belonging to other Enterobacteriaceae. Sensitivity, specificity, and accuracy of the FISH technique were 100%, 86.67%, and 92% respectively with Confidence Interval of 95% (82.5% to 98.4%). Both PNA-FISH and conventional culture technique were used for examination of 100 non-heat-treated milk samples which were artificially contaminated with E. coli O157:H7. PNA-FISH sensitivity, specificity, and accuracy were 97%, 100%, and 98.5% respectively with a Confidence interval of 95% (95.7% to 99.7%). The PNA-FISH technique had approximate results to the conventional culture methods, in addition, it reduced the diagnosis time to one day.

DOI

10.21608/ejah.2025.409052

Keywords

E. coli O157:H7, PNA-FISH, conventional culture method

Volume

5

Article Issue

1

Related Issue

52137

Issue Date

2025-01-01

Receive Date

2024-10-09

Publish Date

2025-01-01

Page Start

100

Page End

110

Print ISSN

2735-4938

Online ISSN

2735-4946

Link

https://ejah.journals.ekb.eg/article_409052.html

Detail API

http://journals.ekb.eg?_action=service&article_code=409052

Order

409,052

Type

Original researches

Type Code

1,636

Publication Type

Journal

Publication Title

Egyptian Journal of Animal Health

Publication Link

https://ejah.journals.ekb.eg/

MainTitle

Estimation of the sensitivity of a novel technique for the detection of E. coli O157 in non-heat-treated milk

Details

Type

Article

Created At

01 Feb 2025