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407887

Expression Profile of NLRP3 Inflammasome in Peripheral Blood Mononuclear Cells and Plasma Interleukin-22 Level in Multiple Sclerosis

Article

Last updated: 09 Apr 2025

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-

Tags

Host response

Abstract

Background: Multiple sclerosis (MS) is a long-lasting neurodegenerative disorder that primarily impacts young adults. Interleukin-22 (IL-22) and the nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome play critical roles in host innate immunity and have been linked to several autoimmune diseases. Objectives: The objectives of our work are to assess the plasma levels of IL-22 and the expression of the NLRP3 inflammasome in peripheral blood mononuclear cells (PBMCs) in MS patients versus healthy controls. Furthermore, to correlate the level of plasma IL-22 and NLRP3 inflammasome expression in MS patients. Methodology: The study examined 40 patients with MS and 40 matched healthy controls. It measured plasma IL-22 using enzyme-linked immunosorbent assay (ELISA) and assessed NLRP3 expression through real-time reverse transcription polymerase chain reaction (RT-PCR). Results: Patients with MS exhibited markedly elevated levels of plasma IL-22 (P < 0.0001) and a significantly elevated relative expression of NLRP3 (P < 0.0001). Additionally, a significant positive correlation was observed between plasma IL-22 and NLRP3 relative expression (r = 0.45, P = 0.003). Also, ssignificant positive correlations were noted between plasma IL-22 level and both the Expanded Disability Status Scale (EDSS) and frequency of relapses in the past year (r = 0.49, 0.55, P-values = 0.001, 0.0002 respectively). Similarly, EDSS and frequency of relapses in the past year were positively correlated with NLRP3 relative expression (r = 0.4, 0.69, P-values = 0.01, < 0.00001 respectively). Conclusion: Both IL-22 and NLRP3 inflammasome could be encouraging prognostic markers and potential therapeutic targets for MS. 

DOI

10.21608/ejmm.2025.352611.1441

Keywords

EDSS, Interleukin-22, Multiple sclerosis, NLRP3, Inflammasome

Authors

First Name

Heba

Last Name

Elbatal

MiddleName

M.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University

Email

hebamosadelbatal@kasralainy.edu.eg

City

Cairo

Orcid

0000-0001-5353-408X

First Name

Karim

Last Name

Ibrahim

MiddleName

A.A.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University

Email

dr.karimabdo@gmail.com

City

Cairo

Orcid

-

First Name

Zeinab

Last Name

Mostafa

MiddleName

A.I

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University

Email

zeinabmostafa61@gmail.com

City

-

Orcid

-

First Name

Amr

Last Name

Fouad

MiddleName

M.

Affiliation

Neurology Department, Faculty of Medicine, Cairo University

Email

amro.fouad@kasralainy.edu.eg

City

Cairo

Orcid

-

First Name

Asmaa

Last Name

Hegab

MiddleName

S.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University

Email

asmahgab@yahoo.com

City

Ctiro

Orcid

-

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2025-01-13

Publish Date

2025-04-01

Page Start

277

Page End

284

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 2_Pages 277-284.pdf

PDF . 545.4kB

Link

https://ejmm.journals.ekb.eg/article_407887.html

Detail API

http://journals.ekb.eg?_action=service&article_code=407887

Order

33

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Expression Profile of NLRP3 Inflammasome in Peripheral Blood Mononuclear Cells and Plasma Interleukin-22 Level in Multiple Sclerosis

Details

Type

Article

Created At

01 Feb 2025