ManageChatDiscover
407595

Relationship between miR-29a Gene Expression and Progesterone Levels in Iraqi Patients with Breast Cancer

Article

Last updated: 09 Apr 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: In Iraq and around the world, Breast Cancer (BC) is the most frequent disease that affects women. About 22 nucleotides, short and non coding RNA molecules known as microRNAs (miRNAs) are essential for regulating genes and a range of cellular functions, including differentiation, proliferation, and death. The studies have shown that progestogens may bind to both nuclear and membrane receptors, which can stimulate the development of tumors in the breast and increase of cells causing breast cancer. The study was aimed to assess the miRNA-29 expression and the progesterone hormone levels and their association at the patients diagnosed with the breast cancer. Methodology: The number of samples was 74 blood samples, collected from breast cancer patients who were 54 females and apparently healthy subjects (20). The total RNA was extracted from whole blood to synthesis the complementary DNA(cDNA) and level of miR-29a expression was determined by reverse transcription quantitative polymerase chain reaction (RT-qPCR) method. Serum levels of the progesterone hormone were measured by Enzyme linked lmmunosorbent assay (ELISA) method. Results: The Progesterone hormone level  was significantly decreased  in breast cancer patients having a mean equal to (13.27±1.6 ng/ml) in comparison to (20.99±0.8 ng/ml) for the healthy control group (p<0.01) while miR-29a expression was significantly increased in the breast cancer patients (1.2±0.28) in comparison with healthy subjects (0.006± 0.0001) with p value (0.0021). Conclusion: As stated by the findings, the miR-29a expression can be used as diagnostic marker for the breast cancer examination in the laboratory investigations .miR-29 was significantly correlated with progesterone levels in breast cancer. Further studies are needed to  this correlation .

DOI

10.21608/ejmm.2025.352251.1437

Keywords

breast cancer, microRNA, miR 29a, progesterone hormone

Authors

First Name

Dhaneen

Last Name

Mahdi

MiddleName

-

Affiliation

Faculty of Dentistry, University of Kufa

Email

dhaneenmahdi1@yahoo.com

City

najaf

Orcid

-

First Name

Alaauldeen

Last Name

AL-Sallami

MiddleName

S.M.

Affiliation

Faculty of Science, University of Kufa

Email

alaauldeenallami2@yahoo.com

City

najaf

Orcid

-

First Name

Mohammad

Last Name

Alzeyadi

MiddleName

-

Affiliation

Faculty of Science, University of Kufa

Email

mohammadlzeyadi2@yahoo.com

City

najaf

Orcid

-

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2025-01-20

Publish Date

2025-04-01

Page Start

269

Page End

275

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 2_Pages 269-275.pdf

PDF . 379.1kB

Link

https://ejmm.journals.ekb.eg/article_407595.html

Detail API

http://journals.ekb.eg?_action=service&article_code=407595

Order

32

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Relationship between miR-29a Gene Expression and Progesterone Levels in Iraqi Patients with Breast Cancer

Details

Type

Article

Created At

01 Feb 2025