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The biosystematic of E. coli Strains Isolated from Clinical and Environmental Samples Phenotypically and Genotypically in Najaf Governorate

Article

Last updated: 09 Apr 2025

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Subjects

-

Tags

Clinical microbiology

Abstract

Background:  The current work has employed the Clermont phylotyping approach to better elucidate the phylogenetic groupings of E. coli. A new technique called quadruplex PCR has been used to classify Escherichia coli (E. coli) strains into eight different phylogenetic groupings. However, it is still unknown how exactly these bacterial lineages relate to one another phylogenetically. Objectives: This study aimed to isolate, diagnose and classify E. coli bacteria into phylogroups and strains to determine the relationships between isolates taken from clinical and environmental samples in Najaf Governorate. Methodology: Samples collected from Najaf at a rate of 500 samples were subjected to culture, isolation and identification by biochemical tests and PCR technique based on the Clermont classification. Results:  the positive samples were 250 isolates  and seven phylogroups (A, B1, B2, C, D, E and F) were diagnosed in addition to the Unknown group and clade (I, II, V), as well as two pathotype groups (DEC/ EXPEC) and subpathotype groups affiliated with them (APEC, UPEC, SEPEC, MPEC). Also, commensal E. coli was identified. A completely new strain classification was established, and were recorded in the gene bank (NCBI), which classified under the term (Hetero-hybrid pathogenic), as plural of (EXPEC + DEC + DEC). Ten new E. coli isolates were registered in the (NCBI) as a first registration and an extension number was obtained. Conclusion: Using specific diagnostic primers, strains of E. coli bacteria are identified, and virulent strains recorded for the first time in Iraq were diagnosed and a new taxonomic branch designed for this study was adopted.

DOI

10.21608/ejmm.2025.350058.1429

Keywords

Biosystematics, APEC, UPEC, SEPEC, MPEC

Authors

First Name

Waleed

Last Name

Alnasrawy

MiddleName

D.

Affiliation

University of Kufa, Faculty of Sciences, Department of Biology, Najaf - Iraq

Email

waleedd.shamkh@uokufa.edu.iq

City

najaf

Orcid

-

First Name

Mahdi

Last Name

AL-Aammar

MiddleName

H.

Affiliation

University of Kufa, Faculty of Sciences, Department of Biology, Najaf - Iraq

Email

mahdi.alammer@uokufa.edu.iq

City

najaf

Orcid

-

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2025-01-03

Publish Date

2025-04-01

Page Start

259

Page End

267

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 2_Pages 259-267.pdf

PDF . 470.2kB

Link

https://ejmm.journals.ekb.eg/article_407592.html

Detail API

http://journals.ekb.eg?_action=service&article_code=407592

Order

31

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

The biosystematic of E. coli Strains Isolated from Clinical and Environmental Samples Phenotypically and Genotypically in Najaf Governorate

Details

Type

Article

Created At

01 Feb 2025