Background: Multiple Sclerosis is a progressive inflammatory neurological disease affecting central nervous system that may lead to long term disability and even death especially among young adults. So, digital nursing intervention is crucial in providing information about disease, identify needs, improve daily activities and treatment adherence among patients with multiple sclerosis Aim: to assess the effect of digital nursing intervention on performance of daily living activities and treatment adherence among adult patients with multiple sclerosis.. Design: Quasi-experimental (case and control group- pre/ posttest) research design was utilized for the present study. Setting: The current study was conducted at Neurological outpatient clinic at University Hospital in Shebin El –Kom, Menofia Governorate, Egypt. Sample: A purposive sample of 80 adult patients diagnosed with multiple sclerosis was taken in this study. Instruments: Three instruments were used I: Structured interviewing questionnaire to gather socio demographic data, patient's medical data, and Patients' knowledge regarding multiple sclerosis, II: Modified Barthel Index scale, and III: Multiple Sclerosis Treatment Adherence Questionnaire. Results: After three and six months of application of digital nursing intervention, total mean score of knowledge among the study group was increased to 14.6 and18.2-compared to 9.1 and 9.8 in the control group respectively. Also, The mean score of performance of Daily Living activity was improved to 15.82±4.24 and 17.07±3.96 in the study group compared to 8.07±3.79 and 8.67±4.28 in the control group respectively. In addition, the study group had a statistical significant improvement in in all item of treatment adherence at post 3 and 6 months of intervention compared to control group (P<0.001).. Conclusion: Digital nursing intervention ascertained its efficacy in increasing knowledge, enhancing performance of daily living activities and improving treatment adherence of patients with multiple sclerosis. Recommendation: Creation of Web site under supervision of medical and nursing to increase information about the disease and facilitate follow up of patients with multiple sclerosis.