Background: Thalassemia is the commonest hereditary hemolytic anemia in Egypt. It was estimated that 1000 per 1, 5 Million live births per year are born with thalassemia. Iron overload is a major complication of repeated blood transfusion in patients with thalassemia. Objectives: This study was conducted to evaluate Iron overload in pediatric patients with beta thalassemia major and comparing this  with  the  control serum level. Methods: The study included fourty five patients with ß- thalassemia major with fourty five age and sex matched controls. Patients were selected from the outpatient's clinics of the Pediatric Hematology unit and the inpatients of Pediatric department. Patients and control were  subjected  to  clinical  evaluation and complete blood count along with  serum  ferritin  (using  the  ELISA technique). Results: serum ferritin level was significantly higher in patients with beta thalassemia major compared with controls. There was significant difference between both groups as regard , TIBC (µM), HbA2 level (%), HbA level (%), HbF level (%). Conclusion: We concluded that the serum levels of ferritin were significantly higher in patients with beta thalassemia major in comparison to control group.