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405495

EFFICACY OF FREEZE-DRIED INACTIVATED VACCINE AGAINST RABBIT ENTEROTOXAEMIA

Article

Last updated: 20 Jan 2025

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Subjects

-

Tags

Microbiology, mycology and immunology

Abstract

Clostridium (C.) perfringens type A is a leading cause of enterotoxemia in rabbits, significantly affecting outcomes in rabbit farming. It results in a high mortality rate, particularly in weaned rabbits. Vaccination is the most effective control method for this disease. The study focused on preparing and assessing a rabbit bloat vaccine of inactivated toxoid of C. perfringens type A in a lyophilized form that is reconstituted at the time of vaccination using saponin that acts as an adjuvant. The immune response and keeping quality of the prepared vaccine were evaluated in rabbits every 6 months till 24 months of storage at 4 ᵒC and compared to a local vaccine prepared from an inactivated toxoid of C. perfringens type A using aluminium hydroxide gel as an adjuvant. The results showed that all vaccinated rabbits had protective antibodies against C. perfringens, and the results from tested rabbits achieved 4 IU/ml for C. perfringens types A α toxin. It was also found that the lyophilized vaccine prepared from the toxoid of C. perfringens type A gave a higher antitoxic titer than the local vaccine, which lasted protected for up to 24 months; in contrast, the local vaccine lasted only up to 12 months. It was clear that using the lyophilization method not only affects the potency of the vaccine but also increases the duration of preservation of the vaccine and reduces the time spent during vaccine transportation. Also, using saponin as an adjuvant helps in stimulating the immune response.

DOI

10.21608/avmj.2025.323070.1407

Keywords

enterotoxaemia vaccine, lyophilization, saponin

Authors

First Name

ELHAM

Last Name

EL-SERGANY

MiddleName

F.

Affiliation

Department of Anaerobic Bacterial Vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Agricultural Research Center (ARC), Abbasia, Cairo Egypt.

Email

elham1832@hotmail.com

City

Cairo

Orcid

-

First Name

EL-HELW

Last Name

H.A.

MiddleName

-

Affiliation

Department of Anaerobic Bacterial Vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Agricultural Research Center (ARC), Abbasia, Cairo Egypt.

Email

hamedelhelw@hotmail.com

City

Cairo

Orcid

0000-0003-2851-9632

First Name

ABDALLA

Last Name

Y.A.

MiddleName

-

Affiliation

Department of Anaerobic Bacterial Vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Agricultural Research Center (ARC), Abbasia, Cairo Egypt.

Email

yasseradawy@gmail.com

City

Cairo

Orcid

-

First Name

MARWA

Last Name

AHMED

MiddleName

M.

Affiliation

Department of Anaerobic Bacterial Vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Agricultural Research Center (ARC), Abbasia, Cairo Egypt.

Email

zidanmarwa@gmail.com

City

Cairo

Orcid

0000-00015814-5286

First Name

NOURA

Last Name

KHALAF

MiddleName

M.

Affiliation

Department of Anaerobic Bacterial Vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Agricultural Research Center (ARC), Abbasia, Cairo Egypt.

Email

nooramoh1982@gmail.com

City

Benha

Orcid

0009-0005-9024-2859

First Name

ALIAA

Last Name

ABD EL-BAKEY

MiddleName

-

Affiliation

Department of Anaerobic Bacterial Vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Agricultural Research Center (ARC), Abbasia, Cairo Egypt.

Email

vet.alia2008@gmail.com

City

Giza

Orcid

-

Volume

71

Article Issue

184

Related Issue

52949

Issue Date

2025-01-01

Receive Date

2024-10-26

Publish Date

2025-01-01

Page Start

643

Page End

651

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 71_Issue 184_Pages 643-651.pdf

PDF . 510.1kB

Link

https://avmj.journals.ekb.eg/article_405495.html

Detail API

http://journals.ekb.eg?_action=service&article_code=405495

Order

405,495

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

EFFICACY OF FREEZE-DRIED INACTIVATED VACCINE AGAINST RABBIT ENTEROTOXAEMIA

Details

Type

Article

Created At

20 Jan 2025