The current study aimed to determine the infection rate of Salmonella abortusovis, Brucella spp, and Listeria monocytogenes among ewes in Babylon Province. For this purpose blood samples were collected from 50 aborted ewes, 15- 30 days after abortion, and 10 normal deliveries of ewes during the period from September 2021 to June 2022. The collected samples were evaluated by using ELISA. The results showed that from the aborted cases, 78% (39/50 cases) were positive for one or more of the diseases examined, while regarding the 11 normal deliveries, only 27% (3 out of 11) were found positive. Estimation of infection rate among the aborted ewes using Anti-Salmonella abortusovis antibody, anti-Brucella spp antibody and anti-Listeria monocytogenes antibody detected positive cases at rates of 66%, 22% and 12% respectively, and 25.6% of cases gave antibody titre against more than one etiology that was subjected in the current study. The current study reveals that 78.7% of abortions caused by Brucella spp., took place during the third semester.