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404827

Study on the Efficacy of Allogeneic Bone Marrow Derived Mesenchymal Stem Cells in Induction of Acute Myeloid Leukaemia Blasts Maturation

Article

Last updated: 20 Jan 2025

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Abstract

Aacute myeloid leukemia (AML) is a severe hematological malignancy with a poor prognosis. Although chemotherapy remains the standard treatment, its effectiveness is limited, with high rates of relapse. Stem cell therapy has emerged as a promising alternative, yet its potential for AML prevention and treatment requires further investigation. This study aimed to evaluate the efficacy of bone marrow-derived mesenchymal stem cells (BM-MSCs) in promoting the maturation of AML blasts. Peripheral blood samples were obtained from 39 AML patients for the isolation of blast cells, while BM-MSCs were derived from healthy donors. An in vitro co-culture system was employed to study the interactions between BM-MSCs and AML blasts, assessing the proliferative capacity of BM-MSCs and their effects on leukemia cells (LCs). Flow cytometry was used to evaluate the expression of specific markers before and after co-culture with allogeneic BM-MSCs, focusing on the induction of AML blast maturation. The results demonstrated a significant reduction in the expression of CD13 and CD33 markers in AML blasts following co-culture with BM-MSCs across all samples. Specifically, the expression of CD13 decreased from 70.74% to 5.02%, and CD33 expression was reduced from 69.49% to 5.14%, indicating a marked decline in AML blast populations. In conclusion, the findings suggest that BM-MSCs appear to be effective in inducing the maturation of AML blasts, offering a potential therapeutic strategy for AML management.

DOI

10.21608/eajbsz.2025.404827

Keywords

Acute myeloid leukaemia (AML), Bone marrow derived mesenchymal stem cells (BM-MSCs), leukemia cells (LCs)

Authors

First Name

Ramy

Last Name

Ibrahim

MiddleName

B.

Affiliation

Zoology department, Faculty of Science, Suez University, Egypt.

Email

avabavlylab@gmail.com

City

Egypt

Orcid

-

First Name

Hala

Last Name

Gabr

MiddleName

-

Affiliation

Hematology department, Faculty of Medicine, Cairo University, Egypt.

Email

halagabr@yahoo.com

City

Egypt

Orcid

-

First Name

Fadia

Last Name

Atia

MiddleName

M.

Affiliation

Clinical Pathology department Faculty of Medicine, Suez Canal University, Egypt.

Email

-

City

Egypt

Orcid

-

First Name

Aida

Last Name

Hussein

MiddleName

A.

Affiliation

Zoology department, Faculty of Science, Suez University, Egypt.

Email

aida.hussein@sci.suezuni.edu.eg

City

Egypt

Orcid

orcid: 0000-0002-9822-9086

Volume

17

Article Issue

1

Related Issue

52817

Issue Date

2025-06-01

Receive Date

2024-12-03

Publish Date

2025-01-17

Page Start

15

Page End

25

Print ISSN

2090-0759

Online ISSN

2090-0821

Article File

EAJBSZ_Volume 17_Issue 1_Pages 15-25.pdf

PDF . 937.4kB

Link

https://eajbsz.journals.ekb.eg/article_404827.html

Detail API

http://journals.ekb.eg?_action=service&article_code=404827

Order

404,827

Type

Original Article

Type Code

672

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, B. Zoology

Publication Link

https://eajbsz.journals.ekb.eg/

MainTitle

Study on the Efficacy of Allogeneic Bone Marrow Derived Mesenchymal Stem Cells in Induction of Acute Myeloid Leukaemia Blasts Maturation

Details

Type

Article

Created At

20 Jan 2025