This study provides information on nutritional characteristics through changes in the Gastrosomatic index (GI) and Clark in Caragobius urolepis. After one year of research (10/2022-9/2023), 628 individuals (324 males and 304 females) were caught using trawl nets in Dong Hai, Bac Lieu, and Dam Doi, Ca Mau. The results showed that the GI of this fish varied by season (Pseudo-F= 12.17; P< 0.01) and sex (Pseudo-F= 7.47; P< 0.01) and not site (Pseudo-F= 3.50; P= 0.06). The value of Clark index did not vary by sex (Pseudo-F= 0.89; P= 0.76) and site (Pseudo-F=3.26; P= 0.07) but significantly changed by season (Pseudo-F=118.37; P< 0.01). The sex × season interaction did not affect GI and Clark indices, but sex × site significantly affected GI (Pseudo-F= 9.23; P< 0.01), and site × season affected Clark (Pseudo-F= 23.20; P< 0.01). The results of this study contribute to providing additional data on the nutritional characteristics of Caragobius urolepis, with a basis for future artificial breeding research of this species.