Background: Bioremediation of industrial and environmental wastes using thermoalkalistable lipase-producing bacteria offers a sustainable solution for waste degradation and potential applications for various industries.
Objective: This study aimed to isolate and optimize the production of lipase from thermoalkalophilic bacterial strains, making them ideal for industrial processes. Materials and Methods: Agro-industrial waste samples viz. (water hyacinth, lubricant oil waste, frying oil, used sesame oil, and dried chicken skin) were used for isolation of lipase bacterial producers. These samples were enriched under alkaline and elevated temperature conditions (65^oC and pH10). Lipase production was optimized using one factor at a time approach by testing various physical and nutritional factors including temperature, time, pH, inoculum size, additional carbon sources, nitrogen sources, vitamins, metals, and amino acids.
Results: Fourteen isolates were obtained and screened for lipase productivity, the most potent isolate was selected and identified genetically based on 16srRNA as Parageobacillus thermoglucosidasius, SO1 with 100% ident to 100% query. The optimization was effective leading to 151.69% increase of lipase productivity (237.93±0.53U/ml) by Parageobacillus thermoglucosidasius, SO1 at the optimum conditions 70 ^OC, pH 9.5 for 2 days with 8% inoculum size and 2% sesame oil with the addition of 1% sucrose, 0.76% DL-Tyrosine, 200ppm MnSO₄ and 200ppm folic acid.
Conclusion: The study demonstrates that optimizing process significantly enhanced lipase production, making the selected bacterial strain suitable for different industrial applications as detergent industry, where thermostable and alkali-tolerant enzymes are in high demand.