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405701

Impact of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Coupled with Antimicrobial Stewardship Program on Pediatric ICU Patients in

Article

Last updated: 09 Apr 2025

OverviewSimilar Items

Subjects

-

Tags

prevention of hospital infections

Abstract

Background: Antimicrobial resistance (AMR) poses a significant global threat to public health and safety. To address this challenge, antimicrobial stewardship programs (ASPs) play an important role in optimizing antimicrobial use, reducing adverse effects, and lowering healthcare costs. Integrating advanced diagnostics like MALDI-TOF MS enhances ASP effectiveness, potentially improving antimicrobial use, lowering AMR rates, and bettering patient outcomes. Objective: This study aimed to evaluate the clinical impact of implementing MALDI‐TOF MS alongside ASP on the outcomes of PICU patients with various infections at Cairo University Specialized Pediatric Hospital (CUSPH). Method: A prospective cohort study was conducted in the PICUs at CUSPH. The 13-month study was divided into three phases: pre-implementation (baseline assessment), implementation (establishing guidelines and ASP orientation), and post-implementation. Key ASP indicators, such as length of stay, cure rate, mortality rate, length of therapy, and days of therapy, were measured. Results: In the post-implementation phase, both the average length of stay and mortality rate showed a notable decrease, with p-values of 0.059 and 0.06, respectively, suggesting a trend toward significance. The cure rate increased (p = 0.06). Additionally, the use of amphotericin significantly decreased (p = 0.03), while the use of cefepime (p = 0.002), clindamycin (p = 0.01), and linezolid (p = 0.03) increased. Klebsiella pneumoniae was the most isolated organism, accounting for 21% of cases. Conclusion: Integrating MALDI-TOF MS with ASP significantly reduce time to organism identification, improving time to optimal antibiotics therapy, with positive outcome measures including shortening hospital stays, increasing cure rate, and lowering mortality rates.

DOI

10.21608/ejmm.2025.351290.1430

Keywords

Antimicrobial resistance, Antimicrobial stewardship programs, Matrix-assisted laser desorption-ionization time of flight mass spectrometry, Cairo University Specialized Pediatric Hospital

Authors

First Name

Hamsah

Last Name

Yousef

MiddleName

A.

Affiliation

Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University

Email

hamsahyousef123@gmail.com

City

Cairo

Orcid

0009-0008-6316-0366

First Name

Sahar

Last Name

Khairat

MiddleName

M.

Affiliation

Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University

Email

sahar.khairat@gmail.com

City

Cairo

Orcid

-

First Name

Mona

Last Name

Abdelhalim

MiddleName

M.

Affiliation

Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University

Email

mona.mohiedden@kasralainy.edu.eg

City

Cairo

Orcid

0000-0001-7852-3045

First Name

Sally

Last Name

Ishak

MiddleName

K.I.

Affiliation

Department of Pediatrics, Faculty of Medicine, Cairo University

Email

sallykamal2034@gmail.com

City

Cairo

Orcid

0000-0002-5212-131X

First Name

Elshimaa

Last Name

Ahmed

MiddleName

S.

Affiliation

Department of Pediatrics, Faculty of Medicine, Cairo University

Email

elshimaasalah@yahoo.com

City

Cairo

Orcid

0000-0002-3823-0667

First Name

Sherifa

Last Name

Salem

MiddleName

T.

Affiliation

Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University

Email

cherifa_tarek@kasralainy.edu.eg

City

Cairo

Orcid

0000-0003-2303-0619

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2025-01-07

Publish Date

2025-04-01

Page Start

217

Page End

224

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 2_Pages 217-224.pdf

PDF . 371.0kB

Link

https://ejmm.journals.ekb.eg/article_405701.html

Detail API

http://journals.ekb.eg?_action=service&article_code=405701

Order

26

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Impact of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Coupled with Antimicrobial Stewardship Program on Pediatric ICU Patients in

Details

Type

Article

Created At

20 Jan 2025