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404831

Impact of Neutrophil Extracellular Trapping on Hypervirulent Klebsiella pneumoniae in Type 2 Diabetes Mellitus

Article

Last updated: 09 Apr 2025

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Tags

Clinical microbiology

Abstract

Background: There is increasing evidence that type 2 diabetes mellitus (T2D) patient has higher risk for hypervirulent Klebsiella pneumoniae (hvKP) infections. Neutrophils achieve different immunological functions such as phagocytosis, and neutrophil extracellular trap (NETs) formation to arrest and eradicate microbes such as hvKp. Physicians have restricted curative approach for treatment of infection due to these superbugs. This necessitates to more understand innate immune arm included in these infections. Objective: This study aimed to investigate in vitro interaction of human NETs (as one of innate immune components) with hvKp in T2D. Methodology: Twenty patients with T2D and twenty age and sex matched healthy persons as healthy controls (HCs) were included in this study. By using clinical-derived hvKp strains, NETs complex testing by immunofluorescence, phagocytosis detection and NETs killing analysis were performed. Results: Direct killing of diabetic patients NETs against hvKp strain significantly decreased compared to NETs of HCs despite NETosis induction was significantly higher in neutrophils of T2D patients, compared to neutrophils of HCs. HvKp showed significantly lower level of phagocytosis compared to the control strain (14.65% vs. 96.43%). However, insignificant difference in the level of phagocytosis was detected between T2D and HCs neutrophils. Conclusion: Our study suggests that impaired NETs bactericidal ability makes diabetic patient more susceptible to develop hvKp invasive infection. This will shed light on a novel NETs dependant therapeutic approach and improved therapeutic plan against hvKP infection in T2D patient.

DOI

10.21608/ejmm.2025.349387.1423

Keywords

Neutrophil extracellular traps, Hypervirulent Klebsiella pneumoniae, Type 2 diabetes mellitus

Authors

First Name

Lamiaa

Last Name

Salama

MiddleName

A.

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Horus University, Egypt; College of Pharmacy, Uruk University, Baghdad, Iraq

Email

-

City

-

Orcid

-

First Name

Elshimaa

Last Name

Eleraky

MiddleName

S.

Affiliation

Internal Medicine Department, Faculty of Medicine, Horus University, Egypt

Email

-

City

-

Orcid

-

First Name

Iman

Last Name

Fawzy

MiddleName

M.

Affiliation

Mansoura Central Laboratories, Clinical Pathology Department, Ministry of Health, Egypt

Email

-

City

-

Orcid

-

First Name

Ziad

Last Name

Selim

MiddleName

Y.

Affiliation

Mansoura Manchester Medical Program, Faculty of Medicine, Mansoura University, Egypt

Email

-

City

-

Orcid

-

First Name

Safaa

Last Name

Elageery

MiddleName

M.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Egypt

Email

safageery@gmail.com

City

-

Orcid

-

First Name

Noha

Last Name

Abou El-Khier

MiddleName

T.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Egypt

Email

-

City

-

Orcid

-

First Name

Nawal

Last Name

Gouda

MiddleName

S.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Egypt; Medical Microbiology and Immunology Department, Faculty of Medicine, Northern Border University, Saudi Arabia

Email

-

City

-

Orcid

-

First Name

Rasha

Last Name

Elnagar

MiddleName

M.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt; Basic Medical Sciences Department, College of Medicine, Al-Maarefa University, P.O.Box71666, Riyadh 11597, Saudi Arabia

Email

drrasha_m@mans.edu.eg

City

-

Orcid

0000-0002-5661-850X

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2024-12-31

Publish Date

2025-04-01

Page Start

189

Page End

194

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 2_Pages 189-194.pdf

PDF . 352.9kB

Link

https://ejmm.journals.ekb.eg/article_404831.html

Detail API

http://journals.ekb.eg?_action=service&article_code=404831

Order

22

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Impact of Neutrophil Extracellular Trapping on Hypervirulent Klebsiella pneumoniae in Type 2 Diabetes Mellitus

Details

Type

Article

Created At

20 Jan 2025