The organizational support and psychological empowerment of nurses can be impacted by the civility of their first line managers. Aim: to examine the effect of the civility educational program on first line managers' psychological empowerment and Organizational Support. Research design: Quasi-experimental research design. Setting: The research was applied at kidney and urology university Hospital in Minia City. Subjects: All staff nurses who worked in the same departments as the head nurses were employed in the inpatient and outpatient units, their total numbers were (220) nurses were classified as follow: head nurses (20) and staff nurses (200). Tools: four tools were used in this study; civility knowledge questionnaire, workplace civility climate Self-Assessment scale, psychological empowerment scale and organizational support scale. Results: The total knowledge score and levels of perceived workplace civility among head nurses were low pre than post implementation of the program, they had higher scores with statistically significant differences; also, the staff nurse psychological empowerment score and organizational support score were increased. Conclusions: The first line manager educational program had positively affected their knowledge as well as self-assessment about perceived workplace civility post program implementation with statistically significant variations and the nurse psychological empowerment and perceived organizational support levels were increased Recommendation: Periodical first line manager programs and seminars must enhance their civility competencies, behaviors, as well as communication to motivate and empower their nurses and being organizationally supported.