Background: Workers in slaughterhouses engaging in unhygienic practices create conducive environments for zoonoses and meat contamination. Knowledge of hygiene practices and their determinants provides evidence for the design of targeted interventions. Aim: To assess occupational health hazards prevalence, knowledge, attitude and practice among butchers at Assiut District and City. Subjects and method; Study design: A community-based descriptive, cross-sectional design was used. Setting: Butchers' shops in Assiut District and City. Sample: Included 350 people working in butcher profession: Study tools: Three tools were included in this study; Tool (I): A structured interview sheet consisted of five sections; personal characteristics, work related data, work environment assessment, reported occupational health problems, and butchers' knowledge. Tool (II): Butchers' attitude regarding occupational health hazards. Tool (III): Butchers' practice observational checklist. Results: It was found that 45.1% of butchers aged<40 years, 40.9% had secondary education, 45.7% had fair level of knowledge with significance relation with education, residence, and nature of work. Also, 78.9% of them had a positive attitude with positive links with education, residence and receiving training courses and 70.0% of them had satisfactory practices which correlated with education, residence and work experience. Conclusion: Butchers had a fair level of knowledge, positive attitude and satisfactory level of practices regarding occupational hazards. Recommendations: Health education program regarding occupational safety and the importance of protective personal equipment should be encouraged.