Background: Helicobacter pylori (H. pylori) is a virulent pathogen causing gastritis and ulcers followed by serious complications. Nurse's role in this assessment is vital for empowering students to make informed decisions about their health and well-being. Aim: This study aimed for assessment of El-Minya nursing institute students' knowledge, practices and attitude regarding helicobacter pylori disease. Research design: Descriptive research design was used in this study. Sample: Convenient sample included 157 nursing institute students. Setting: Minya nursing institute, Minya governorate. Tool for data collection: One tool, Interview questionnaire included five parts, 1st part: Socio-demographic characteristics of nursing students, 2ndpart: Past and current medical history of students, 3rd part: Nursing institute's students' knowledge, 4th part: Nursing students' attitude about H. pylori disease and 5th part: Nursing student's reported practice questionnaires. Results: 83.4 % of nursing institute student had poor total knowledge. 60.5 % of them had unsatisfactory total reported practice and 79.9 % of them had negative total attitude regarding helicobacter pylori disease. Conclusion: Majority of the studied nursing institute students had poor total knowledge, also less two third of them had unsatisfactory total reported practices, and more two third of them had negative attitude regarding H. pylori disease.. Recommendations: Design health education program for nursing institute students' knowledge, practices and attitude which include all information about helicobacter pylori disease.