Mastitis, an inflammation of the mammary gland, can be clinical or subclinical; it is one of the most important multifactorial pathologies. The current research aimed to evaluate the impact of a symbiotic on subclinical mastitis in dairy cattle and its effect on milk quality. Two milk samples were taken from two groups of dairy cows of different breeds, particularly Montbéliard, Flekvi, and Holstein, all fed the same ration. The cows were divided into two groups: an experimental group of 18 cows that received the symbiotic “SYMBIOVEBA®" as an alternative treatment and 10 cows served as a control group. Subclinical mastitis was assessed using the California Mastitis Test (CMT), and milk quality was analyzed with the Lactoscan. The symbiotic's impact on preventing as well as reducing subclinical mastitis in dairy cattle, and on milk quality was evaluated. According to the obtained results, the symbiotic caused a modification in the composition of milk, characterized by a non-significant increase in butyrous content. These results suggest that the use of the symbiotic has a positive impact on subclinical mastitis. The change in milk composition, with a non-significant increase in butyrate, may indicate an improvement in udder health and a reduction in the symptoms of subclinical mastitis. This research highlights the potential importance of using symbiotics as an alternative treatment for subclinical mastitis and providing a foundation for future studies and interventions to improve dairy cattle health and milk quality