This study aimed to evaluate the effect of dried stinging nettle plant parts (roots, leaves, seeds) on performance, egg quality, and some biochemical serum parameters. The experiment used 96 Hy-Line W-36 layers, of 28 weeks of age, and was randomly divided into four dietary treatments: the first group fed a diet without nettle as a control (CG), the second group (RG) fed a diet treated with 1% nettle roots, the third group (LG) fed a diet treated with 1% nettle leaves, and the fourth group (SG) fed a diet treated with 1% nettle seeds. Each experimental unit used four replicates, six layers each. The density of 595 cm² / bird. The experimental period lasted 16 weeks. The results demonstrated that supplementing 1% roots, leaves, or seeds of stinging nettle to layers diets did not show any significant differences in egg production, egg mass, egg weight, feed intake, and feed conversion ratio (FCR). Likewise, yolk%, albumen%, eggshell%, yolk index, and Haugh units, serum levels of total protein, total cholesterol, triglyceride, glucose, AST, and ALT, as well, egg yolk cholesterol concentration did not show significant differences (P>0.05). Generally, the use of 1% of stinging nettle parts, such as roots, leaves, and seeds did not improve performance, egg quality, and biological serum parameters.