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400838

CHARACTERIZATION, ANTIMICROBIAL PROFILE, AND BIOFILM FORMATION OF ACINETOBACTER SPECIES ISOLATED FROM MILK AND HUMAN

Article

Last updated: 13 Jan 2025

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Subjects

-

Tags

Microbiology, mycology and immunology

Abstract

One hundred milk samples were obtained from diverse places in Qena City, and fifty urine samples were gathered from patients with Urinary Tract Infections (UTIs) attending various laboratories in Qena City. All the samples were examined for the presence of Acinetobacter species on Leeds Acinetobacter Medium (LAM). The suspected colonies were identified through Gram staining and biochemical tests. The recA gene was identified using polymerase chain reaction (PCR), which was used for the molecular identification of the Acinetobacter species, and the identified species were further characterized by sequencing this gene. Phylogenetic analysis of the Acinetobacter species was then conducted. The identified Acinetobacter species were tested for antibiotic susceptibility and their ability to produce biofilm. The results of this study showed that 20 out of 150 samples were positive for Acinetobacter species using conventional isolation methods. Using PCR techniques, 18 samples were positive for Acinetobacter. Sequencing of the recA gene for the 18 isolates revealed 7 Acinetobacter species, which were identified as 3 A. baumannii, 1 A. nasocomialis, 1 A. urisingii, and 2 A. johnsonii. The antibiotic susceptibility testing showed that most Acinetobacter species were multi-antibiotic resistant (MAR) and harbored antibiotic resistance genes. The results of biofilm formation testing, using both the Congo red and microtiter plate methods, revealed that most Acinetobacter isolates were strong biofilm producers. In conclusion, the presence of Acinetobacter species which are highly resistant to antibiotics especially A. baumannii in milk and urine samples may threaten public health.

DOI

10.21608/avmj.2024.314231.1359

Keywords

Acinetobacter, Milk, rec A, PCR, DNA sequencing

Authors

First Name

HALA

Last Name

SAMIR

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, South Valley University 83523 Qena, Egypt.

Email

halasamir155@gmail.com

City

-

Orcid

-

First Name

HAMS

Last Name

MOHMED

MiddleName

MA

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, South Valley University 83523 Qena, Egypt.

Email

drhams85@yahoo.com

City

-

Orcid

-

First Name

MOHAMED

Last Name

ABD EL-AZEEM

MiddleName

WAEL

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, South Valley University 83523 Qena, Egypt.

Email

wael_2000uk@yahoo.co.uk

City

-

Orcid

-

First Name

WALEED

Last Name

YOUNIS

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, South Valley University 83523 Qena, Egypt.

Email

wkhrdr39@gmail.com

City

qena

Orcid

-

Volume

71

Article Issue

184

Related Issue

52949

Issue Date

2025-01-01

Receive Date

2024-08-21

Publish Date

2025-01-01

Page Start

128

Page End

145

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 71_Issue 184_Pages 128-145.pdf

PDF . 1.0MB

Link

https://avmj.journals.ekb.eg/article_400838.html

Detail API

http://journals.ekb.eg?_action=service&article_code=400838

Order

400,838

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

CHARACTERIZATION, ANTIMICROBIAL PROFILE, AND BIOFILM FORMATION OF ACINETOBACTER SPECIES ISOLATED FROM MILK AND HUMAN

Details

Type

Article

Created At

13 Jan 2025