The current study was designed with an aim to evaluate immune staining expression of IFN-γ and IL-15 in patients with AA (between lesional and non-lesional skin biopsies) assessing their relationship to clinical parameters of the diseases. It was a case-control study, including thirty-six patients with localized, recent onset AA, a 3.5 mm punch skin biopsies were taken from lesional and non-lesional skin 5 cm away from the lesion of every patient as a control group for immunohistochemical analysis of IL15 and IFN-γ. The level of IL15 and IFN-γ show correlation with the severity of inflammation as they were higher in the more severe cases and who has multiple lesions. They show non-statistically differences between healthy skin biopsy and lesional skin biopsy. There were neither affected by the subject's age and site and extent of lesion. We can conclude that skin of severe cases of AA has a high level of IL15 and IFN-γ than localized and mild cases