The research was conducted during October 2022 to May 2023 with the aim of studying the fish Biodiversity in the Al-Bireh Dam- Hama- Syria. Fish samples were collected using Gills  nets and Hooks. The results showed the presence of the species Aphanius vladykovi, which is recorded for the first time in the Syrian fresh waters. This species is characterized by the upper mouth, the eye in the upper side of the head, and the presence of smooth teeth on the jaws. The end of the lateral line is dark black, there are no spines, and rays of fins are soft. The ventral area has a light color, while the dorsal area is dark. Light spots are spread on the body of the fish, and the scales are firmly attached to the skin.