The purpose of the research was to improve the morpho-anatomical and physiological features of somatic embryos (SEs) of date palms in tissue culture media that have the potential to produce green and healthy plantlets. Copper sulfate (CuSO₄) and cobalt chloride (CoCl₂) were supplemented to date palm maturation medium at various concentrations, separately or combined. Adding CoCl₂ to the media of maturation produced the greatest relative growth and differentiation percentages of date palm embryogenic calli, particularly at 0.52 mgL^-1. In comparison to the control, when combined treatments were administered alone, the quantity of the produced somatic embryos rose dramatically (17/jar). Total sugars and free amino acids were the highest in SEs grown on a maturation medium with CoCl₂ at 0.26 mgL^-1. Moreover, total phenols and indoles were picked significantly at their higher levels in the generated SEs that were cultured on a medium enhanced with 0.52 mgL^-1. When the maturation medium was administered along with both as a treatment, the antioxidant enzyme, ascorbate peroxidase, reached its maximum activity. Whereas, adding 0.52 mgL^-1 of CoCl₂ to the culture medium enhanced the activities up to of highest levels of ascorbate peroxidase,  polyphenol oxidase and phenylalanine ammonia-lyase.