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400882

Morphological, cytogenetic and biochemical studies on the effects of zinc oxide in bulk form vs the nanoprepared form as fertilizers on Pisum sativum L. plant.

Article

Last updated: 13 Jan 2025

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Abstract

Zinc element is very essential in planting process; supplying plants with zinc element improve plant growth, development and performances in norm and under stresses. The new introduced nano zinc preparations should be evaluated and revised for their safety usage in agriculture. Methods: in this work the zinc oxide fertilizer in naturally bulk form is used as positive control to visualize effect of zinc oxide in nano form in different concentrations (50, 100 & 200ppm) on Pisum sativum plant. The study concerned some morphological traits and the total soluble seedling's protein profile after the soil around root was injected with used materials, in addition to cytogenetic studies on the mitosis system after the direct treatment for 3hr and after the 24hr of recovery experiment. Results: the study confirms the great risk of the nanoforms usage on all the studied parameters on the level of the two higher concentrations if compared to -ve and +ve control. It induced lowering in all the morphological parameters. On the level of cytogenetic parameters, it empathized its chromo-toxic effect on the DNA physical constituent and mutagenic effect via induction of micro/ macronucleus in resting cells.  It also affected the gene expression via disappearance of some protein bands from image. Conclusion: lower concentration of nano zinc oxide particles can be used to enrich new reclaimed lands in limited conditions (if necessary) to avoid its mutagenic harm on plant as recommended alternative and to evade the risk of soil suffocation with  excess ZnO fertilizer in bulk form.

DOI

10.21608/jsrs.2024.337476.1140

Keywords

Pisum sativum L, ZnO NPs, plant growth, mitosis apparatus, Protein profile

Authors

First Name

Safaa

Last Name

Saleh

MiddleName

Mohamed

Affiliation

Botany Department, Faculty of Women For Arts, Science and Education Ain Shams University, Cairo, Egypt.

Email

safaaismael28@gmail.com

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Orcid

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First Name

Nahla

Last Name

O.M.Ehsan

MiddleName

-

Affiliation

Botany Department, Faculty of Women For Arts, Science and Education Ain Shams University, Cairo, Egypt.

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Orcid

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First Name

Thoria

Last Name

R. Mohamed

MiddleName

-

Affiliation

Botany Department, Faculty of Women For Arts, Science and Education Ain Shams University, Cairo, Egypt.

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Orcid

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First Name

Rania

Last Name

T. Ali

MiddleName

-

Affiliation

Genetics and Cytology Department, National Research Center, Dokki, Egypt.

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Orcid

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First Name

Emad

Last Name

A. Abdeldaym

MiddleName

-

Affiliation

Vegetable Crops Department, Faculty of Agriculture, Cairo University, Giza, Egypt.

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City

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Orcid

-

First Name

Abd-Elwahab

Last Name

M. Mahmoud

MiddleName

-

Affiliation

Physiology Department, Faculty of Agriculture, Cairo University, Giza, Egypt.

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Orcid

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Volume

41

Article Issue

2

Related Issue

52534

Issue Date

2024-12-01

Receive Date

2024-11-19

Publish Date

2024-12-01

Page Start

95

Page End

121

Print ISSN

2356-8364

Online ISSN

2356-8372

Link

https://jsrs.journals.ekb.eg/article_400882.html

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http://journals.ekb.eg?_action=service&article_code=400882

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400,882

Type

Original Article

Type Code

656

Publication Type

Journal

Publication Title

Journal of Scientific Research in Science

Publication Link

https://jsrs.journals.ekb.eg/

MainTitle

Morphological, cytogenetic and biochemical studies on the effects of zinc oxide in bulk form vs the nanoprepared form as fertilizers on Pisum sativum L. plant.

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Type

Article

Created At

13 Jan 2025