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404387

Correlation between Methicillin Resistance Gene Presence, Biofilm Production and Antibiotics Pattern of Staphylococcus aureus Wound Isolates and the Activity of Possible River Wa

Article

Last updated: 09 Apr 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Wound infections with Staphylococcus aureus strains, a potential health care problem, especially after methicillin-resistant Staphylococcus aureus (MRSA) occurrence, which poses a significant challenge for healthcare workers. Objective: is to identify the relationship between the existence of the gene mecA within isolated bacterial cells and the phenomenon of multiple antibiotic resistance profiles of these strains. The study also explored the potential therapeutic activity of river water bacteriophage. Methodology: Among other bacterial species isolated from wound infections, 34 strains of S. aureus were isolated. After the screening for mecA gene using the Polymerase Chain Reaction Technique (PCR), the antibiotic susceptibility profiles of S. aureus isolates were determined by the disk diffusion method to identify the relationship between the two parameters. Furthermore, the possibility of Bacteriophage existence against isolated S. aureus was investigated. Results: A strong correlation between the existence of the gene mecA and the phenomenon of multiple antibiotic resistance profiles, particularly beta-lactams as well as commonly prescribed antimicrobial agents was present. Moreover, the results of the bacteriophage spots showed the possible presence of bacteriophage against S. aureus wound isolates. Conclusion: There was significant relationship between bacterial possessing of gene mecA, their biofilm production ability and their multidrug resistant phenomenon. River water bacteriophage could be used to treat pathogen bacteria included in this study.

DOI

10.21608/ejmm.2025.341642.1393

Keywords

MRSA, antibiotics, mecA genes, Staphylococcal aureus, Wound infections

Authors

First Name

Sarah

Last Name

Abdulsattar

MiddleName

A.H.

Affiliation

Microbiology Department, College of Medicine, University of Anbar, Ramadi, Iraq

Email

sar21m0003@uoanbar.edu.iq

City

Ramadi

Orcid

-

First Name

Mohammed

Last Name

Alfeehan

MiddleName

J.

Affiliation

Surgery Department, College of Medicine, University of Anbar, Ramadi, Iraq

Email

mjmohammed@uoanbar.edu.iq

City

Ramadi

Orcid

-

First Name

Mohammed

Last Name

Mohaisen

MiddleName

R.

Affiliation

Department of Basic Sciences, College of Dentistry, University of Anbar, Ramadi, Iraq; Department of Clinical Infection, Microbiology and Immunology, Institute of Infection, Veterinary, and Ecological Sciences, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, L69 7TX, United Kingdom.

Email

den.m.radhi@uoanbar.edu.iq

City

Ramadi

Orcid

orcid.org/0000-0002-0329-752X

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2024-12-04

Publish Date

2025-04-01

Page Start

175

Page End

181

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 2_Pages 175-181.pdf

PDF . 489.8kB

Link

https://ejmm.journals.ekb.eg/article_404387.html

Detail API

http://journals.ekb.eg?_action=service&article_code=404387

Order

20

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Correlation between Methicillin Resistance Gene Presence, Biofilm Production and Antibiotics Pattern of Staphylococcus aureus Wound Isolates and the Activity of Possible River Wa

Details

Type

Article

Created At

13 Jan 2025