ManageChatDiscover
404131

Activity of Nigella Sativa Oil on Multidrug Resistant Organisms Isolated from Diabetic Foot Patients

Article

Last updated: 09 Apr 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Multidrug-resistant (MDR) organisms are becoming more prevalent worldwide, which is an issue in poor nations that depend on natural resources, especially traditional and herbal plant sources. Nigella sativa oil (NSO) is a strong antibacterial that could be an excellent option. Objective: The aim of the present work is to determine the in vitro antibacterial efficacy of NSO against MDR microbes isolated from diabetic foot ulcers. Methodology: By using the agar well diffusion method to evaluate the zone of inhibition of NSO, the antibacterial impact of NSO against MDR bacteria of diabetic foot ulcers was ascertained. The tube dilution method was used to find the minimal inhibitory concentration (MIC), and sub-culturing tubes that showed no discernible growth or turbidity in the MIC allowed for the determination of the minimal bactericidal concentration (MBC).  Results:  NSO produced zone of inhibition ranged from 0 mm. to 28 mm., MIC ranged from 3% to 100% and MBC ranged from 6% to 100% against different MDR bacteria of diabetic foot ulcer. Bacterial outcomes and inhibition zones made by NSO against different isolated organisms were insignificantly different between Gram-positive and Gram-negative organisms. Conclusion: NSO had antibacterial effects against MDR Gram-positive and Gram-negative organisms. It showed significant synergism in combination with different antibiotics. 

DOI

10.21608/ejmm.2025.345941.1405

Keywords

diabetic foot ulcer, Nigella Sativa, zone of inhibition, Synergism

Authors

First Name

Mohamed

Last Name

Abd El-Hamid

MiddleName

I.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Tanta University, Tanta, Egypt

Email

mohamed.mohamed2@med.tanta.edu.eg

City

-

Orcid

-

First Name

Marwa

Last Name

Abd-Elmonsef

MiddleName

M.E.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Tanta University, Tanta, Egypt

Email

marwa.ezzat@med.tanta.edu.eg

City

Tanta

Orcid

0000-0002-7779-0405

First Name

Emad

Last Name

Sallam

MiddleName

M.

Affiliation

Vascular and Endovascular Surgery Department, Faculty of Medicine, Tanta University, Tanta, Egypt

Email

emadsallam81@yahoo.com

City

-

Orcid

-

First Name

Sara

Last Name

Abd El Galil

MiddleName

I.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Tanta University, Tanta, Egypt

Email

dr.roka.sara@gmail.com

City

-

Orcid

-

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2024-12-19

Publish Date

2025-04-01

Page Start

169

Page End

174

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 34_Issue 2_Pages 169-174.pdf

PDF . 310.6kB

Link

https://ejmm.journals.ekb.eg/article_404131.html

Detail API

http://journals.ekb.eg?_action=service&article_code=404131

Order

19

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Activity of Nigella Sativa Oil on Multidrug Resistant Organisms Isolated from Diabetic Foot Patients

Details

Type

Article

Created At

13 Jan 2025