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403578

Genetic Variants of EGFR and EpCAM in association with HCC Susceptibility in HBV Infected Iraqi Patients

Article

Last updated: 13 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Genetics

Abstract

Background: Primary liver cancer (PLC) is a highly lethal malignancy and the third most common cause of tumor-associated death annually. Hepatocellular carcinoma (HCC) accounts for 75–85% of all PLC cases. Hepatitis B virus (HBV) remains a major risk factor for HCC. HBV share important functions in the (EGFR) signaling pathway, with EpCAM potentially serving as a cancer stem cell marker in HCC. Objective: to assess whether EGFR and EpCAM gene variants in HBV patients in Iraq contribute to the development of HCC. Methodology: A total of 120 samples were collected, comprising 40 HCC patients (group I), 40 chronic HBV (CHB) patients (group II), and 40 healthy controls (group III). SNPs identification was done using the polymerase chain reaction-HRM (PCR-HRM) curve analysis. Results Genotyping rs884225 (EGFR gene), both genotype and allele frequencies show no statistically significant differences between HCC, CHB patients, and HCs (all p-values > 0.05). In contrast, rs62139665 in the EpCAM gene showed significant associations. The AA genotype and A allele were strongly linked to HCC (p < 0.01) and moderately to CHB (p < 0.05). The AA genotype demonstrated a very high odds ratio for HCC (OR = 10.7), while the A allele showed a moderate association with CHB (OR = 1.8). Conclusion: The AA genotype and the A allele of rs62139665 in the EpCAM gene are potential genetic markers for susceptibility to HCC and CHB, whereas rs884225 in the EGFR gene does not significantly contribute in the risk for these diseases in Iraqi population

DOI

10.21608/ejmm.2025.346646.1412

Keywords

HCC, HBV, eGFR, EpCAM, PCR-HRM

Authors

First Name

Khalid

Last Name

Majeed

MiddleName

R.

Affiliation

Department of Medical Laboratory Technique, Al-Nasiriyah Technical Institute, Southern Technical University, Thi-Qar, Iraq

Email

khalid.gen12@stu.edu.iq

City

Thi-Qar

Orcid

0000-0001-7002-4372

First Name

Wiaam

Last Name

AL-Amili

MiddleName

A.

Affiliation

Department Genetic Engineering/ Institute of Genetic Engineering and Biotechnology for Postgraduate Studies / University of Baghdad / Baghdad / Iraq

Email

dr.wiaam@ige.uobaghdad.edu.iq

City

Baghdad

Orcid

0000-0002-6434-3147

First Name

Safaa

Last Name

Al-Waysi

MiddleName

A.A.

Affiliation

Medical City, Hepatology and Gastroenterology Teaching Hospital

Email

safaalwaisy@gmail.com

City

Baghdad

Orcid

0009-0009-5191-0059

Volume

34

Article Issue

2

Related Issue

51523

Issue Date

2025-04-01

Receive Date

2024-12-24

Publish Date

2025-04-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_403578.html

Detail API

http://journals.ekb.eg?_action=service&article_code=403578

Order

403,578

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Genetic Variants of EGFR and EpCAM in association with HCC Susceptibility in HBV Infected Iraqi Patients

Details

Type

Article

Created At

13 Jan 2025