Background: Children on hemodialysis are at excessive liability to Hepatitis C Virus because of repeated blood get admission to and scientific devices. Nurses play a crucial role in infection prevention, but deficiencies in their skills and knowledge might compromise patient care. Aim: To assess nurses performance regarding application of preventive measures of hepatitis C virus infection among children undergoing hemodialysis. Design: This study was conducted using an exploratory, descriptive design. Setting: This study was carried out in the Hemodialysis Units at Sohag University Hospitals. Sample: A convenience sample comprising all 50 nurses employed in hemodialysis units. Tools:To gather data, two tools were used. Tool (I): Structured interview questionnaire for nurses.Tool (II): Checklists for Practice Observation. Results: The results indicate that, 56% of nurses lacked sufficient knowledge about how to apply hepatitis C preventive methods. The results indicate that 38% of nurses in the study applied preventive measures against infection with the hepatitis C virus in children receiving hemodialysis competently, where as 62% of nurses in the study applied these measures incompetently. The total particle level of the nurses under study and their educational background were statistically significantly correlated. Conclusion: According to the study, over half of the nurses lacked adequate information about Hepatitis C virus prevention. Additionally, less than half of nurses demonstrated competent practices in implementing these measures. Recommendation: Implementing health educational programs and training to enhance nurses' knowledge and skills in preventing Hepatitis C infections within pediatric hemodialysis units.