The goal of this study was to assess the antimicrobial activity of various extracts of medicinal plants seed as lepidium sativum, coriandrum sativum, and cuminum cyminum against pathogenic strains, G+ bacteria , and G- bacteria . As determined the mold strains and the yeast strain was Candida albicans , by the well diffusion assay, The findings revealed that hexane extracts of Lepidium sativum had the efficacy against all tested strains except G+ bacteria. The highest restricting zone of Coriandrum sativum extracts appeared with ethanolic (2.6mm), methanolic (2.03mm), and petroleum ether (1.7mm) extracts against A. flavus. The petroleum ether extract of Cuminum cyminum shown antibacterial activity against A. niger, A. flavus, and Macro phaseoli mold, with zones of inhibition measuring 2.03, 2.22, and 1.3 mm. Overall, all extracts had a considerable impact against strain A. flavus. In contrast, strains A. niger and Macro. phaseoli were resistant to all extracts of Cuminum cyminum and Coriandrum sativum. It is concluded that, the hexane extract of Lepidium sativum shown effective antibacterial activity against tested strains and is regarded as a potential extract for further research and applications. It is advised to follow this study's findings, which demonstrate that Lepidium sativum hexane extracts exhibited the biggest zone of inhibition in comparison to other extracts like methanol and ethanol. Novel medications to treat a range of microbial infections in humans may be developed using Lepidium sativum as a raw material. Consequently, the research suggests adding Lepidium sativum during foods preparing for improve quality and safety diet