The present trial investigated the efficacy of dietary curcumin nanoparticles on reproductive performance, haemato-biochemical parameters, antioxidants, immunity and in vitro embryo production of rabbit does. Mature rabbit does (APRI line) were distributed into 4 groups. Does in the first treatment were fed commercial diet (CP). The rabbit in 2nd, 3rd, and 4th were fed CP with curcumin nanoparticles at a levels of 3,5, and 7 mg per kg diets respectively. All does were naturally mated with fertile APRI bucks (five bucks per treatment). Results showed that does in all treatments showed significantly (P<0.05) better in vivo reproductive efficiency in terms of rates of conception and kindling rate and litter size, as well as total cholesterol, triglycerides, high and low density lipoproteins as a lipid profile markers , antioxidant status such as concentrations of total antioxidant capacity, glutathione, and malondialdehyde, and activity of glutathione peroxidase, glutathione S-transferase, superoxide dismutase and catalase), immunity and ovulatory response of CLs number and ovulation rate), in vitro embryo quality, and hatched blastocysts production as compared to other treatments. In conclusion, dietary supplementation with curcumin nanoparticles had positive effects on in vivo and in vitro reproductive efficiency of doe rabbits.