Background: It's time to wait two weeks once the embryo transfer is finished. Most IVF patients dread this period of time. They saw the doctor almost daily up until this point. They will now need to wait 10–14 days before they are able to take a pregnancy test. It's critical that patients minimize stress and resume their regular life as much as possible during the two-week wait. Aim: to determine the effect of nurse-led instructional protocol on practices and pregnancy rate of women during waiting period after embryo transfer. Methods: Research design: quasi experimental design was used to carry out the study. Setting: Private infertility center (El-Nada center), Menoufia governorate. Sample: non-probability convenient sampling of 88 women were recruited for this study. Instruments: three instruments were used for data collection a) structured interviewing questionnaire schedule b) Knowledge assessment questionnaire c) Practice assessment questionnaire. Results: According to this study, there was a substantial difference between the groups' post-intervention pregnancy rates, women's knowledge levels, and certain healthy practices. Conclusion: Implementation of nurse-led instructional protocol was efficient in enhancing knowledge, practices, and pregnancy outcomes among women during waiting period after embryo transfer. Recommendations: Infertile women should be adequately informed about the waiting period following embryo transfer, and the use of such a nurse-led instructional strategy should be mandated to improve assisted reproductive technology (ART) outcomes.