Background: The monkeypox virus causes the infectious illness known as "human monkeypox infection," which has significant health hazards for the public. The two major goals of the global response to the monkeypox outbreak were to improve knowledge and attitudes as well as to use preventative measures to stop the disease from spreading further. Aim of the study: was to evaluate the effect of nano-teaching sessions regarding human monkeypox infection on knowledge, attitude and preventive measures among pregnant women. Subjects and Methods: Research design: This study used a quasi-experimental design. Setting: The research was carried out at the prenatal care unit in Al-Nahal medical center that provides healthcare services to the population of Zagazig, Egypt. Subjects: 70 pregnant women were included in a purposive sample. Tools of data collection: three tools were used; a structured Interviewing questionnaire, attitude questionnaire regarding human monkeypox infection among pregnant women and preventive measures questionnaire regarding human monkeypox infection among pregnant women. Results: There was a highly statistical significant correlation between pregnant women's preventive measures and their knowledge at pre and post nano-teaching sessions and their attitude after implementation of nano-teaching sessions regarding human monkeypox infection. Conclusion: Implementation of nano-teaching sessions had a highly statistically significant effect on improving pregnant women's knowledge, attitude and preventive measures toward Human monkeypox Infection. Recommendations: Continuous campaigns to improve women's knowledge, attitude, and preventive measures are crucial because controlling outbreaks requires the substantial cooperation of a knowledgeable and aware society.