ManageChatDiscover
391457

Characterization of Antimicrobial Activity and Fingerprinting for Disorganized and Organized Cultures in Datura innoxia

Article

Last updated: 13 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Biochemistry

Abstract

Datura innoxia is renowned for its pharmaceutical qualities and traditional medical use. It's crucial for long-term pharmaceutical drug synthesis by regulated micropropagation and tissue culture techniques. This study focused on establishing Datura innoxia under aseptic laboratory settings to promote callus formation and comparing its phytochemical profile with leaves using High-Performance Liquid Chromatography (HPLC). The goal was to identify potential changes in phytochemical content between various growing strategies. Antibacterial efficacy of extracts from conventional plants and lab-grown callus were evaluated against Escherichia coli and Staphylococcus aureus strains. Moreover, aiming to ascertain the genetic stability of Datura innoxia cells in different conditions, the fingerprinting profile was performed by Start Codon Targeted (SCoT) and Inter-Simple Sequence Repeat (ISSR) markers. By thoroughly examining these features, the study provides insights into the biochemical, pharmacological, and genetic properties of Datura innoxia, which are critical for its use in pharmaceutical research and development. These findings highlight the critical role of regulated cultivation practices in improving the efficacy and sustainability of medicinal plant-based therapies.

DOI

10.21608/ejchem.2024.316633.10318

Keywords

Datura innoxia, tissue culture, HPLC, antimicrobial activity, SCoT, ISSR, fingerprinting

Authors

First Name

Mohamed

Last Name

El-Awady

MiddleName

A. M.

Affiliation

Genetics Department, College of Agriculture, Cairo University, Giza, Egypt.

Email

mohamed_elawady@yahoo.com

City

-

Orcid

-

First Name

M.

Last Name

Abdel-Hady

MiddleName

S.

Affiliation

Biotechnology Group, Botany Dept., National Research Centre, Dokki, Giza, Egypt.

Email

sarwat_sayed@yahoo.com

City

-

Orcid

0009-0002-9319-9436

First Name

Alia

Last Name

Hegazy

MiddleName

M.

Affiliation

Biotechnology English Program, Faculty of Agriculture, Cairo University, Giza, Egypt.

Email

aliaamostafa852002@gmail.com

City

-

Orcid

0009-0008-3850-346X

First Name

Dina

Last Name

Samy

MiddleName

H.

Affiliation

Biotechnology English Program, Faculty of Agriculture, Cairo University, Giza, Egypt.

Email

dinahany1165@gmail.com

City

-

Orcid

0009-0003-6351-8261

First Name

Habibatullah

Last Name

Rashad

MiddleName

H.

Affiliation

Biotechnology English Program, Faculty of Agriculture, Cairo University, Giza, Egypt.

Email

habiba.beba200@gmail.com

City

-

Orcid

0009-0005-0112-4886

First Name

Habiba

Last Name

Shahat

MiddleName

-

Affiliation

Biotechnology English Program, Faculty of Agriculture, Cairo University, Giza, Egypt.

Email

habibashahatm12@gmail.com

City

-

Orcid

0009-0002-3787-2008

First Name

Mayar

Last Name

Mohamed

MiddleName

W.

Affiliation

Biotechnology English Program, Faculty of Agriculture, Cairo University, Giza, Egypt.

Email

mayarwaleed133@gmail.com

City

-

Orcid

0009-0002-8406-3254

First Name

Minas

Last Name

Samha

MiddleName

K.

Affiliation

Biotechnology English Program, Faculty of Agriculture, Cairo University, Giza, Egypt.

Email

minassamhaa12@gmail.com

City

-

Orcid

0009-0009-3488-4039

First Name

Esmaeel

Last Name

Salah

MiddleName

M.

Affiliation

Biotechnology Department, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo.

Email

esmaeel.bio@azhar.edu.eg

City

-

Orcid

0009-0006-8587-8761

Volume

67

Article Issue

13

Related Issue

46555

Issue Date

2024-12-01

Receive Date

2024-09-01

Publish Date

2024-12-01

Page Start

1,863

Page End

1,871

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 67_Issue 13_Pages 1863-1871.pdf

PDF . 635.2kB

Link

https://ejchem.journals.ekb.eg/article_391457.html

Detail API

http://journals.ekb.eg?_action=service&article_code=391457

Order

391,457

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Characterization of Antimicrobial Activity and Fingerprinting for Disorganized and Organized Cultures in Datura innoxia

Details

Type

Article

Created At

13 Jan 2025