Strawberry became one of the most important fruit crops in last Decade. Strawberry infected with many pathogens and one of the most important pathogens in Egyptian fields is wilt disease. In this study there are twenty isolates of wilted strawberry. The causal pathogen isolates identified as Fusarium oxysporum. Pathogenicity tests carried out with the isolated strains of F. oxysporum shown wilt symptoms. Strawberry used for symptoms incidence F. oxysporum and revealed wilt disease; chromium nanoparticle was evaluated under greenhouse conditions, Uniform fungicide used as standard control. Chromium nanoparticle shown success results in controlling the wilt causal pathogen Fusarium oxysporum.