Diabetes mellitus is a serious metabolic disease. Diabetic nephropathy (DN) is a common consequence of diabetes. Quercetin, in particular, has been extensively examined and shown to have positive therapeutic effects on various human diseases, including diabetes. It has significant anti-diabetic benefits, such as lowered blood sugar levels and enhanced insulin sensitivity. It can also prevent and improve nephropathy one of the diabetes complications. This study aims to inspect the prophylactic effect of Quercetin in struggling DN following T1DM development in rats. T1DM was generated in the experimental rats by delivering a single high dose of STZ (65 mg/kg.bw) intraperitoneally, followed by ten weeks of Quercetin therapy. Serum of Diabetic rats showed high levels of urea, creatinine, Na, and K, indicating microvascular issues. Oxidative stress was clearly present, along with an increased inflammatory response. Diabetic rats' kidney tissue showed a significant rise in TNF-α, TGB, and amount of IL-1β. Renal morphological changes were found in all groups following the introduction of T1DM, quercetin significantly improved. Biochemical markers like fasting blood glucose (FBG), insulin, urea, and creatinine can reduce the incidence of TNF-α, IL-6, and TGB. Quercetin professionally protects the kidney and reduces injury of renal tissue via lowering free radical damage and reestablishing glucose homeostasis, suggesting a possible line of therapy for T1DM nephropathy.