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403188

Analysis of immunodiagnostic potency of excretory secretory antigens of Haemonchus contortus by SDS-PAGE

Article

Last updated: 07 Jan 2025

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Abstract

Background: Helminthic excretory secretory (E/S) antigens gained considerable interest due to their
role in stimulating host-parasite interactions and as potential candidate for vaccine development.
Objective: To quantify and characterize H. contortus E/S antigens aiming for its use as an effective control
measure.
Material and Methods: Adult worms were collected from the abomasa of slaughtered goats. Fresh and
highly motile adult worms were transferred to RPMI 1640 culture medium containing antibiotics. The
E/S antigen was procured through standard protocols and concentration of proteins was measured.
Characterization of ES antigen was subjected to SDS-PAGE and estimation of protein concentration.
Results: The triturate contained proteins with a concentration of 0.836 mg/ml. Three bands of proteins
appeared on the gel with molecular weights of 65, 48 and 30 kDa.
Conclusion: Further characterization of E/S antigens will be helpful in developing effective control
measures against the worms in general and haemonchosis specifically.

DOI

10.21608/puj.2024.313940.1265

Keywords

E/S antigens, goats, H. contortus, Immunodiagnosis, Nematodes, SDS-PAGE

Authors

First Name

Zubaria

Last Name

Amin

MiddleName

-

Affiliation

Departments of Livestock and Dairy Development, Animal Science Institute, Manager Production, Hi Tech Feed and Oil Mills, Quetta, Balochistan

Email

-

City

-

Orcid

-

First Name

Muhammad

Last Name

Imran

MiddleName

-

Affiliation

Departments of Parasitology, Faculty of Veterinary Science, University of Agriculture ,Manager Production, Hi Tech Feed and Oil Mills, Quetta, Faisalabad

Email

-

City

-

Orcid

-

First Name

Asim

Last Name

Shamim

MiddleName

-

Affiliation

Faculty of Veterinary and Animal Sciences , Manager Production, Hi Tech Feed and Oil Mills, Azad Kashmir3, Pakistan

Email

asimshamimuajk@gmail.com

City

-

Orcid

-

First Name

Muhammad

Last Name

Sajid

MiddleName

-

Affiliation

University of Agriculture , Manager Production, Hi Tech Feed and Oil Mills, Quetta Faisalabad ,Pakistan

Email

drsohailuaf@hotmail.com

City

-

Orcid

-

First Name

Hafiz

Last Name

Rizwan

MiddleName

-

Affiliation

Manager Production, Hi Tech Feed and Oil Mills, Quetta , Lahore ,Pakistan

Email

hm.rizwan@uvas.edu.pk

City

-

Orcid

-

First Name

Tanzeel

Last Name

Rehman

MiddleName

-

Affiliation

Manager Production, Hi Tech Feed and Oil Mills, Quetta ,Sahiwal , Pakistan

Email

-

City

-

Orcid

-

First Name

Syed

Last Name

Shah

MiddleName

-

Affiliation

Faculty of Veterinary and Animal Sciences, University of Poonch , Pathobiology (Parasitology)section, Manager Production, Hi Tech Feed and Oil Mills, Quetta ,Rawalakot, Azad Kashmir , Pakistan

Email

-

City

-

Orcid

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Volume

17

Article Issue

3

Related Issue

52791

Issue Date

2024-12-01

Receive Date

2024-08-20

Publish Date

2024-12-01

Page Start

211

Page End

213

Print ISSN

1687-7942

Online ISSN

2090-2646

Article File

PUJ_Volume 17_Issue 3_Pages 211-213.pdf

PDF . 325.3kB

Link

https://puj.journals.ekb.eg/article_403188.html

Detail API

http://journals.ekb.eg?_action=service&article_code=403188

Order

7

Type

Original Article

Type Code

426

Publication Type

Journal

Publication Title

Parasitologists United Journal

Publication Link

https://puj.journals.ekb.eg/

MainTitle

Analysis of immunodiagnostic potency of excretory secretory antigens of Haemonchus contortus by SDS-PAGE

Details

Type

Article

Created At

07 Jan 2025