Tuna loin is a high-value fishery commodity with growing demand, but its sustainability is threatened by various challenges. Analyzing the configuration of the tuna loin supply chain in Maluku Province, Indonesia, is crucial for understanding the role of each actor and for identifying opportunities for improving business processes to enhance efficiency and sustainability. This research aimed to analyze the tuna loin agroindustry supply chain using four frameworks proposed by Van der Vorst: chain structure, chain management, chain resources, and business processes. In Maluku Province, six distinct patterns of tuna loin agroindustry supply chain networks were identified. However, the dominant patterns are Type 1 (traditional fishermen → collectors/mini plants → processing companies → consumers) and Type 2 (traditional fishermen → processing companies → consumers). The partnership in the supply chain involves fishermen, collecting traders, processing companies, and exporters, and is built on mutual benefit and trust, which enhances the efficiency and sustainability of tuna production. The success of the tuna loin supply chain in Maluku depends heavily on physical resources, such as fishing vessels and freezing facilities, as well as the quality of human resources, including skills, knowledge, and collaboration among stakeholders. The business processes in the supply chain involve relationships between members, seen through two perspectives: cycle view and push/pull view. The findings from this research provide valuable insights for improving chain management in the tuna loin agroindustry and assist supply chain stakeholders in developing strategies to promote sustainable practices in the supply chain.