The present study aimed to investigate the impact of dietary supplements on some biochemical variables (lactate, creatine kinase CK, lactate dehydrogenase LDH, and ammonia) in heptathlon athletes in relation to their athletic performance. The sample consisted of 5 athletes who had been selected to participate in the study. An experimental approach was used through a pre-post comparison of an experimental group that received dietary supplements. Significant improvements in some of these biochemical variables were observed in the study sample, indicating the role of supplements in enhancing athletic performance and accelerating muscle recovery. In this regard, the study recommended the addition of dietary supplements to the nutritional regimen of heptathlon athletes, although determining the appropriate dosage for each individual athlete is necessary. Further studies are also needed to determine the physiological mechanisms by which dietary supplements affect these biochemical variables and overall athletic performance.