ABSTRACT
Objective: To assess the impact of bone marrow mesenchymal stem cells (BMMSCs) and their derived exosomes on inducible nitric oxide synthase (iNOS) levels in cisplatin (CP) induced parotid salivary gland damage.
Methods: Cryopreserved BMMSCs line with cell density 106 was used in this study. BMMSCs derived exosomes were purchased from Nawah Scientific lab. Fifty-six male albino rats in good health weighting from 200-250 g were allocated into 4 groups (n=14); group I; rats received phosphate buffered saline (PBS), group II; rats were intraperitoneally injected with CP, group III; were injected with CP then after 3 days they were injected with BMMSCs via tail vein and group IV; received CP and after 3 days they were injected with BMMSCs-exosomes via tail vein. After 7 and 14 days, scarification of animals were done, and the parotid glands were collected and processed to be examined histologically and immunohistochemically using (iNOS) immune marker.
Results: Groups treated with BMMSCs, and exosomes showed marked decrease in the percentage of iNOS immunohistochemical positive reaction which was significantly lower in the group treated with exosomes in comparison to the group that received BMMSCs.
Conclusions: BMMSCs-exosomes have a potent effect in reducing the inflammatory effect of CP on rats' parotid salivary gland.