Background: Subacromial impingement syndrome (SIS) is the most frequent condition affecting the shoulder. Although there is little evidence about the relation between shoulder pain, disability and axial thoracolumbar vertebral rotation in patients with SIS. Objective: This study aimed to determine the relation between shoulder pain, disability and axial thoracolumbar vertebral rotation in cases of SIS. Patients and methods: A total of 34 Individuals of both sexes with a mean age of 27.76 ± 6.15 years and a mean BMI of 25.16 ± 2.82 kg/m² were recruited for the study with a diagnosis of unilateral SIS. Shoulder pain and disability levels were assessed by shoulder pain and disability index (SPADI), while axial thoracolumbar vertebral rotation was assessed using a scoliometer. Results: Current research revealed that the mean of axial thoracolumbar vertebral rotation for all subjects was 5.09 ± 0.93 degrees with the minimum and maximum rotation found as 4 and 7 degrees respectively and this was considered within normal values, also there was no significant correlations between pain, disability and total score of SPADI and axial thoracolumbar vertebral rotation Conclusion: Patients with SIS had a normal axial thoracolumbar vertebral rotation.