The field experiment was carried out during 2020/ 2021and 2021/ 2022 seasons on Washington navel orange   trees and grown in a private citrus orchard located at Wady Al-Molak, district Al-Ismailia Governorate, Egypt. Used randomized complete block design with three replicates. The results showed that significant between treatments for all studies characters.
The treatment (5 L. Bio. + 70 kg. N/fed) gave the highest leaf length, leaf width and leaf area. The highest values of N, P and K % obtained from the trees fertilized by resulted from the trees fertilized by (5 L. Bio. + 100 kg. N/fed), treatment in first and second seasons respectively.
In addition, the same treatments gained the highest values of Fe (ppm  ), Mn (ppm ), Zn (ppm , chlorophyll A (mg/100g), chlorophyll B (mg/100g), total chlorophyll (mg/100g) and carotene (mg/100g), in the first and second seasons.
Conclusively, from these results it could  be a combination of chemical and bio fertilizer is not only beneficial in improving the properties and environment of soils, but also promotes growth, macro and micro nutrients, chlorophyll contents in orange. Here, our work confirmed that was helpful for citrus growth.