Background: Acetamiprid is now a public health problem, it's critical to investigate how hazardous it is to the epididymis and seminal vesicles. Therefore, using biochemical and histological analyses, we aimed to assess the harmful effects of sub-acute acetamiprid exposure on the epididymis and seminal vesicles in adult albino rats.

Methods: The study included 28 adult male albino rats, were divided equally into: Group I (negative control group): received only regular diet and tab water orally. Group II (positive control group): gavaged orally with distilled water. Group III (Acetamiprid 1/5 of LD50): gavaged orally with acetamiprid at a dose of 40 mg/kg/day) dissolved in distilled water and group IV (Acetamiprid 1/20 of LD50) gavaged orally with acetamiprid at a dose of 10 mg/kg/day) dissolved in distilled water for 4 weeks. LD50 of acetamiprid 200 mg/kg in water.

Results: Acetamiprid administration significantly increased tissue levels of MDA, also significantly decreased tissue levels of TAC. There was non-significant weight decrease of epididymis and seminal vesicles. Histopathological studies revealed that acetamiprid exposure caused epididymal and seminal vesicular tissue damage.

Conclusion: Sub-acute acetamiprid exposure causes significant biochemical and histopathological damage to epididymis and seminal vesicles of adult albino rats.