Abstract
Bovine Rotavirus and Bovine Coronavirus are considered the most important causes
of diarrhea in neonatal calves. May lead to many losses for the breeder and the animal
economy. This work was aimed for isolation and identification of Rota and Corona
viruses at New Valley governorate. During the period from August 2022 to December
2023, 102 fecal samples were collected from diarrheic (n=92) and healthy calves
(n=10) and isolate on Madin Darby bovine Kidney (MDBK), cell line. All positive
samples on tissue culture were examined for presence of BRV and BCoV by using an
antigen Enzyme Linked Immune Sorbent Assay (ELISA). The results was 22 (21.5%)
and 24 (23.5%) for BRV and BCoV, respectively. The prevalence rate of BRV was
highest in the age groups from one day to one month and there is no difference
between male and female but BCoV infection of age up to 1m in female more than
male