Background: Human papillomavirus (HPV) infection is one of the major causes of infection-related cancer worldwide. HPV vaccine is a prophylactic vaccine designed to prevent cervical cancer and other HPV-related cancers. The aim of this study was to assess Perception of university students regarding human papilloma virus vaccine. Design: A descriptive design was used. Setting: the study was conducted at 4 faculties of Ain shams University that chosen by random assignment Sample: A multi stage sample technique was used to recruit 377 students from the four grades of each faculty. Data collection tools: Two tools were used: 1- Structured interviewing questionnaire. 2- Likert -attitude scale: Results: the study showed that more than three quarter (76.1%) of the studied students had poor level of total knowledge about human papilloma virus vaccine. More than half (57.6%) of the studied students had negative total attitude regarding human papilloma virus vaccine. Moreover, there were no statistically significant relation between the type of studied students' faculty and their knowledge  and attitude about human papilloma virus vaccine Conclusion: The most of the studied students had poor level of total knowledge regarding HPV and its vaccine. Finally, there was statistically significant positive correlation between studied students' total knowledge and total attitude. Recommendations: Developing guidelines and brochures to raising awareness about human papilloma virus infection and its vaccine between university students.