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402752

Controlling karish cheese contamination with Escherichia coli using lytic coliphages.

Article

Last updated: 07 Jan 2025

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Subjects

-

Tags

Agricultural Biotechnology.

Abstract

Escherichia coli (E. coli) is considered a major foodborne pathogen. In this study, lytic coliphages were used to control contamination of Karish cheese with E. coli. Five bacteriophages specific to E. coli were isolated and characterized. The isolated phages showed a strong killing potential against E. coli and were selected for their thermal, pH, and UV stability. On the basis of their characteristics, the five isolated coliphages were found to belong to three phage types. These three coliphages were designated ÆEc1, ÆEc2, and ÆEc3. The in vitro experiments showed that coliphages were able to markedly reduce the numbers of E. coli in Karish cheese after 24 hrs. at room temperature and at 4°C. On the other hand, with a reduction in the number of E. coli, a higher number of coliphage was observed. These results support the possibility of using phages to control pathogenic bacteria in foods.

DOI

10.21608/jsasj.2024.402752

Keywords

coliphages, Escherichia coli, Contamination, Bio-control

Authors

First Name

Tarek

Last Name

Elsharouny

MiddleName

H.M.

Affiliation

Microbiology Department Faculty of Agriculture Sohag University Sohag 82524 Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Ahmed

MiddleName

H. M.

Affiliation

Microbiology Department Faculty of Agriculture Sohag University Sohag 82524 Egypt

Email

-

City

-

Orcid

-

First Name

Naglaa

Last Name

Elshamandy

MiddleName

K. F.

Affiliation

Microbiology Department Faculty of Agriculture Sohag University Sohag 82524 Egypt

Email

naglaa.elshamndy@yahoo.com

City

-

Orcid

-

First Name

Adel

Last Name

Hammad

MiddleName

M.M.

Affiliation

Microbiology Department Faculty of Agriculture Minia University Minia Egypt

Email

amhammad2000@yahoo.com

City

-

Orcid

-

First Name

Mazhar

Last Name

Mohamed

MiddleName

D.A.

Affiliation

Microbiology Department Faculty of Agriculture Sohag University Sohag 82524 Egypt

Email

mazhareisawi@agr.sohag.edu.eg

City

-

Orcid

-

Volume

9

Article Issue

2

Related Issue

50401

Issue Date

2024-12-01

Receive Date

2025-01-04

Publish Date

2024-12-01

Page Start

42

Page End

50

Print ISSN

2357-0725

Online ISSN

2735-5578

Article File

JSASJ_Volume 9_Issue 2_Pages 42-50.pdf

PDF . 1.3MB

Link

https://jsasj.journals.ekb.eg/article_402752.html

Detail API

http://journals.ekb.eg?_action=service&article_code=402752

Order

402,752

Type

Research and Review Papers

Type Code

1,734

Publication Type

Journal

Publication Title

Journal of Sohag Agriscience (JSAS)

Publication Link

https://jsasj.journals.ekb.eg/

MainTitle

Controlling karish cheese contamination with Escherichia coli using lytic coliphages.

Details

Type

Article

Created At

07 Jan 2025