The primary output of the apiary industry, whose heritage parallels that of humanity, is honey. Studies on the antibacterial effect of honey have shown that most bacterial pathogens remain sensitive to it, and it was hypothesized that a combination of antibiotics and honey would delay the development of Multi Drug Resistant (MDR) bacteria more effectively than antibiotics alone. The continued misuse of antibiotics provides strong selective pressure, favoring the emergence of antibiotic-resistant mutants. The objective of the current study is to examine the antibacterial role of citrus honey on resistant bacterial pathogens isolated from semen from patients in El Hussien Hospital. Phenolic compounds and flavonoids in the citrus honey extract (CHE) were analyzed using HPLC. Antioxidant action of the extract was done using the DPPH procedure. Moreover, the anti-inflammatory role of the CHE was done using a hemolysis assay. The safety of the extract was assessed by testing through the MTT method versus Vero cells as normal cells. Eleven various phenolic molecules with various concentrations could be seen in the extract. While separation of flavonoids compounds in citrus honey extract revealed the presence of eight different molecules with different levels. Staphylococcus aureus was identified as the most resistant bacterium from semen from Egyptian patients, and its identification was confirmed by molecular identification with the accession number PQ637167.1. The CHE had an antibacterial impact towards S. aureus with a diameter of 2.8±0.7 mm, as well as antioxidant, anti-inflammatory and cytotoxicity with levels of IC₅₀= 4.21 ±0.2 µg/ml, IC₅₀= 12.21 ±0.2 µg/ml, and CC₅₀=18.2±0.7 respectively. The present results suggest the possible in vitro pleiotropic impact of citrus honey for future pharmaceutical applications.