Obesity is abnormal or excessive fat accumulation that may impair health and osteoporosis is a characterized by the loss of bone mass, osteoporosis affects many of females. Chia seeds are a nutrient good source of mineral and vitamin that can be used in a diet to avoid bone diseases such as obesity, rickets and osteoporosis. This research examined the value of chia seeds in diets and how they protected rats from the effects of obesity and osteoporosis. chemical composition of chia seeds moisture, protein, fat, ash, fiber and minerals contents were determined in dry weight (D.w). It contains a high percentage of calcium, potassium, magnesium and phosphorus. The present study carried out on twenty-five female rats (Sprague Dowely) weighing (170 ±5 g). The first group kept as negative control group which fed on basal diet only while groups from (2-5) were induction with high fat diet and injections of dexamethasone once a week at dose (7mg/kg b. wt / rats) for up to four weeks. Group (2) positive group (+ve). Group (3-5) were fed on basal diet that was supplemented with 3%, 6% and 9% chia seeds for 60 days. The results revealed that all of the treated groups showed significant increases in HDL, superoxide dismutase (SOD) and catalase, in addition to the emergence of a significant improvement in urea, uric acid, creatinine, liver function, CH, TG, LDL, VLDL, malondialdehyde (MDA), glucose and insulin, comparing to positive control group. Our results demonstrate that chia seeds powder have beneficial effects on obesity and osteoporosis risk factors.