This study aimed to reach a comprehensive biological evaluation of Jerusalem artichoke (Helianthus tuberoses L.) tubers aqueous extract in adult male albino and to determine its influence on anti-hyperlipidemia protective and sensory properties of chicken and shrimp pops fortified with the extract. Thirty-six adult male albino rats were divided into six groups and treated orally with three concentrations (100, 200, and 300 mg/kg body weight) of H. tuberoses extract. Blood and liver samples were collected to measure the biochemical indicators, protein level, antioxidant parameters, and histopathological examination. Chicken and shrimp pops fortified with the extract were evaluated for their sensory characteristics. The results showed that decrease in liver and adipose tissue weights using H. tuberoses extract as compared with reference drug (Atorvastatin) group, especially at H. tuberoses extract (200mg/kg bwt). Liver aminotransferase enzyme activity (AST and ALT) significantly increased in the control positive group compared to the control negative group. H. tuberosus extract-treated groups. Total count of HB, other blood indices, and platelets count were slightly increased in treated groups with extract as compared with the reference drug (Atorvastatin) group. Antioxidant enzymes activities (GPx and SOD) were observed at the highest activities were observed at H. tuberosus extract 100 , 300 mg/kg bwt. Administration of the extract induced a significant improvement in histological picture of the liver. Sensory evaluation of chicken and shrimp pops fortified with extract showed acceptability related to taste, tenderness, and appearance. H. tuberoses extract could be used as antihyperlipidemic, properties when used as a food supplement