Nanoparticles are compounds with special qualities that are very small in size (less than 100 nm). As a result, nanoparticles pose many different health concerns. Therefore, this study aimed to estimate basil leaf extract's protective effect on nephrotoxicity tempted by zinc oxide nanoparticles (ZnONPs) in male albino rats. Twenty-four male Sprague Dawley rats were divided into four groups. Group 1 rats were given a basal diet for 4 weeks and served as normal control. Group 2 rats were fed on a basal diet+ 500mg/ kg b.w. from leaves extract by gavage) for 4 weeks. Group 3 rats have a single dose of ZnONPs (600 mg/kg i.p.) on the 29^th day. Group 4 rats received a single dose of ZnONPs (600 mg/kg i.p.) on the 29^th day+ 500 mg/ kg b.w. leaf extract via gavage for 4 weeks. At the end of the experiment, blood samples were taken, internal organs were collected, weighted. A complete blood count was performed. Serum was separated to biochemical examination. Basil leaves extract recorded the highest catechein, benzoic, P-OH-benzoic, ellagic and gallic acids, respectively. Serum creatinine, uric acid, urea, zinc, Calcium, magnesium, sodium, potassium and the renal levels of malondialdehyde increased after injection of ZnONPs, reduced hemoglobin, creatinine clearance, zinc's renal activity and antioxidant enzymes. On the other hand, the administration of basil leaves extract improved kidney functions and renal antioxidant enzymes. Therefore, basil leaves extract can be used as a potential preventive agent against ZnONPs induced nephrotoxicity.