Lentil is a functional food, especially due to its high content in proteins and fibers. Obesity is a risk factor for developing several diseases. The present investigation aimed to study the effect of bread fortified with lentil on obesity and some biochemical parameters of rats. Male albino rats (n=40) were divided into two main groups, Group 1, (8 rats) was kept as a negative control group (-ve) and fed on basal diet, while the second group (32 rats) fed on high fat diet (HFD) for 40 days to induce obesity,then divided into 4 sub-groups, each group contained (8 rats), sub-group 1: Rats fed on HFD control group (+ve), sub-group 2: Rats fed on HFD and wheat bread, sub-groups 3 and 4: Rats fed on HFD and bread fortified with lentil (15 and 30%). Six weeks later, blood samples were collected from rats to estimate lipid profiles, liver enzymes, kidney function, antioxidant enzymes and the level of glucose in serum. Histological of liver tissues are also considered. The results of chemical analysis indicated that orange lentil flour contained a higher level of protein and antioxidant activity compared with wheat flour. The results also showed that bread fortified with lentil 30% had a higher level of protein and antioxidant activity compared with wheat bread. The biochemical parameters of obese rats were improved in HFD fed on bread fortified with lentil 30% and 15% compared to the other groups. The results of liver function were supported by histological studies of hepatocytes. The current study confirms that lentil bread plays a role in reducing obesity and related diseases.